植物の環境感覚:刺激受容から細胞応答まで - 文部科学省 科学研究費補助金 新学術領域研究 平成22年度~26年度

徳富 哲

2014年度

原著論文

  • Yoshitake, Y., Yokoo, T., Saito, H., Tsukiyama, T., Quan, X., Zikihara, K., Katsura, H., Tokutomi, S., Aboshi, T., Mori, N., Inoue, H., Nishida, H., Kohchi,T., Teraishi, M., Okumoto, Y. snd Tanisaka, T. The effects of phytochrome-mediated light signals on the developmental acquisition of photoperiod sensitivity in rice. Sci. Rep. 5 : 7709. doi: 10.1038/srep07709 (2015).

  • Kuroi, K., Okajima, K., Ikeuchi, M., Tokutomi, S. and Terazima, M. Transient conformational fluctuation of TePixD during a reaction. Proc. Natl. Acad. Sci. USA. 111, 14764-14769 doi/10.1073/pnas.1413222111 (2014).

  • Sharma, S., Kharshiing, E., Srinivas, A., Zikihara, Z., Tokutomi, S., Nagatani, A., Fukayama, F., Bodanapu, R., Behera, R. K., Sreelakshmi Y. and Sharma, R. A Dominant Mutation in LOV2 Domain Vicinity Impairs Phototropin1 Signaling in Tomato. Plant Physiol. 164, 2030-2044 doi/10.1104/pp.113.232306 (2014).

  • Okajima, K., Aihara, Y., Takayama, Y., Nakajima, M., Kashojiya, S., Hikima, T., Oroguchi, T., Kobayashi, A., Sekiguchi, Y., Yamamoto, M., Suzuki, T., Nagatani, A., Nakasako, M., Tokutomi, S. Light-induced Conformational Changes of LOV1 (Light Oxygen Voltage-sensing Domain 1) and LOV2 Relative to the Kinase Domain and Regulation of Kinase Activity in Chlamydomonas Phototropin. J. Biol. Chem. 289, 413-422 DOI 10.1074/jbc.M113.515403 (2014).

国際学会(招待講演)

  • Tokutomi, S. “Regulation of Ser/Thr Kinase by Light” 2014 Annual World Congress of Molecular and Cell Biology, 25-28 Apr. 2014, Dalian, China.
  • Okajima, K., Nakasako, M., Kashojiya, S. and Tokutomi, S. “Molecular basis for photoactivation of phototropin kinase ” 16th international Congress on Photobiology, 8-12 Sep. 2014, Córdoba, Argentina.




2013年度

原著論文

  • Takaeda, K., Y. Nakasone, K. Zikihara, S. Tokutomi and M. Terazima (2013) Dynamics of the Amino-Terminal and Carboxyl-Terminal Helices of Arabidopsis Phototropin 1 LOV2 Studied by the Transient Grating. J. Phys. Chem., 49, 15606-15613.

  • Kuroi, K., K. Tanaka, K. Okajima, M. Ikeuchi, S. Tokutomi and M. Terazima (2013) Anomalous diffusion of TePixD and identification of the photoreaction product. Photochem. Photobiol. Sci., 12, 1180-1186.

  • Nakasone, Y., K. Zikihara, S. Tokutomi and M. Terazima (2013) Photochemistry of Arabidopsis phototropin 1 LOV1: transient tetramerization. Photochem. Photobiol. Sci., 12, 1171-1179.

  • Hisatomi, O., Takeuchi, K., Zikihara, K., Ookubo, Y., Yoichi Nakatani, Y., Takahashi, F., Tokutomi, S and Kataoka, H. Blue Light-Induced Conformational Changes in a Light-Regulated Transcription Factor, Aureochrome-1. Plant Cell Physiol. 54 93-106 (2013).

総説・解説・著書

  • Shimazaki, K. and, Tokutomi, S. (2013). Diverse responses to blue light via LOV photoreceptors. Plant Cell Physiol., 54, 1-4.

国際学会(招待講演)

  • Kashojiya, S., Okajima, K., Tokutomi, S. (2013) Structural Basis of Phototropin Kinase Activation (The 6th Asia & Oceania Conference on Photobiology), 10-13 November 2013, Sydney, Australia




2012年度

原著論文

  • Okajima, K., S. Kashojiya, S. Tokutomi, (2012) Photosensitivity of kinase activation by blue light involves the lifetime of a cysteinyl-flavin adduct intermediate, S390, in the photoreaction cycle of the LOV2 domain in phototropin, a plant blue light receptor. J. Biol. Chem., 287, 40972-40981.

  • Demarsy, E,. I. Schepens, K. Okajima, M. Hersch, S. Bergmann, J. Christie, K. Shimazaki, S. Tokutomi and C. Fankhauser, (2012) Phytochrome Kinase Substrate 4 is phosphorylated by the phototropin 1 photoreceptor. EMBO J., 31, 3457-3467.

  • Tanaka, K., Y. Nakasone, K. Okajima, M. Ikeuchi, S. Tokutomi and M. Terazima, (2012) Time-Resolved Tracking of Interprotein Signal Transduction: Synechocystis PixD–PixE complex as a Sensor of Light Intensity. J. Amer. Chem. Soc., 134, 8336-8339.

  • Aihara, Y., T. Yamamoto, K. Okajima, K. Yamamoto, T. Suzuki, S. Tokutomi, K. Tanaka and A. Nagatani, (2012) Mutations in the N-terminal Flanking Region of the Blue-light Sensing Domain LOV2 Disrupt its Repressive Activity on the Kinase Domain in the Chlamydomonas Phototropin. J. Biol. Chem. 287, 9901-9909.





2011年度

原著論文

  • Okajima, K., Matsuoka, D. and Tokutomi, S. (2011) LOV2-linker-kinase phosphorylates LOV1-containing N-terminal polypeptide substrate via photoreaction of LOV2 in Arabidopsis phototropin1. FEBS Lett., 585 : 3391-3395.

  • Tanaka, K., Nakasone, Y., Okajima, K., Ikeuchi, M., Tokutomi, S. and Terazima, M. (2011) Light-Induced Conformational Change and Transient Dissociation Reaction of the BLUF Photoreceptor Synechocystis PixD (Slr1694) J.Mol.Biol.. 409 : 773-785.

総説・解説・著書

  • Iwata, T., Tokutomi, S. and Kandori, H. (2011) Light-induced structural changes of the LOV2 domains in various phototropins revealed by FTIR spectroscopy. BIOPHYSICS., 7 : 289-298.

国際学会(招待講演)

  • Okajima, K., Tokutomi, S. (2011) Light regulation of phototropin kinase. (5th Asia and Oceania Conference on Photobiology), 30 July-1 August 2011, Nara




2010年度

原著論文

  • Takayama, Y., Nakasako, M., Okajima, K., Iwata, A., Kashojiya, S., Matsui, Y. and Tokutomi, S. (2011) Light-Induced movement of the LOV2 domain in an Asp720Asn mutant LOV2-Kinase fragment of Arabidopsis phototropin 2. Biochemistry, 50:1174-83.

  • Tanaka, K., Nakasone, Y., Okajima, K., Ikeuchi, M., Tokutomi S. and Terazima M. (2011) A way to sense light intensity: Multiple-excitation of the BLUF photoreceptor TePixD suppresses conformational change. FEBS Lett., 585:786-790.

  • Toyooka, T., Tanaka, K, Okajima, K., Ikeuchi, M., Tokutomi, S. and Terazima, M. (2011) Macromolecular ding Effects on Reactions of TePixD (Tll0078). Photochem Photobiol., 87 : 584-589.

  • Nakasone, Y., Zikihara, K., Tokutomi, S. and Terazima, M. (2010) Kinetics of conformational changes of the FKF1-LOV domain upon photoexcitation. Biophys.J., 99:3831-39.

  • Kawaguchi, Y., Nakasone, Y., Zikihara, K., Tokutomi, S. and Terazima, M. (2010) When is the helix conformation restored after the reverse reaction of Phototropin. J. Amer. Chem. Soc., 132:8838-39.

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